A weekly webinar from ZP answering questions for the week. All questions and answers are anonymized. There is no need to watch live as the webinar is recorded.

1) QUESTION ONE – …wire sensors

2) QUESTION TWO – making immunosensors

3) QUESTION THREE – Making a custom SPE Question One

1. Can the wire biosensors operate at 3.3 V rather than 0.65 V as normally recommended per ZP specifications? Would this alter the calibration curve or the sensitivity of wire biosensor? 2. ATP wire biosensor is this wire sensor still available, also do you need glycerol for this sensor to work for the wire type? 3. Can we get a calcium wire biosensor? What would the cost of this one be for a pack of 4? 4. How many times can you use one of the wire biosensors in solution. Is it similar to the screen-printed electrodes by cleaning off with DI water? 5. Is calcium an amperometric or potentiometric sensor 6. What is the role of increasing voltage for the wire biosensors or screen-printed electrode? Will it help the sensitivity or the detection of the solution? 7. What is the range of detection for the Nitric oxide, ORP, hydrogen peroxide, ATP, Calcium for screen printed and wire sensors 8. What are the recommended current ranges to operate wire sensor: Hydrogen peroxide, Nitric oxide. 9. Do sensors need to be calibrated for each type of solution? For example, if you were to detect hydrogen peroxide added to pbs, DI water, or any other type of solution. Do each need their own calibration curve before they will function for that type of solution? Also, since the Nitric oxide sensor is difficult to calibrate yourself, does the calibration curve online serve as a good basis for measurements? 10. What type of liquid solution is best for calibrating sensors? 11. The calibration curves online, for each product, are these calibrations based on wire sensor or screen-printed wire sensors? 12. How does the Anapot device measure current in chronoamperometry mode based off the change in voltage?