In a recent publication with Swansea University, ZP’s technology was used to quantify bacteria in a bioreaction. Technologies required to produce or extend this technology are linked below.
This paper introduces a novel approach to measuring bacterial growth yield using square wave voltammetry in conjunction with screen-printed electrodes. The method has proven to be a reliable and accurate means of rapidly assessing bacterial growth levels within culturing media. The findings indicate that this combination of sensors and square wave voltammetry is well-suited for applications where quick detection of bacterial growth, such as in a bioreactor, is essential.
Lysogeny broth (LB) (Sigma 12780052) was prepared following the manufacturer’s instructions. The sterile LB was inoculated with a food isolate of Lactobacillus casei and cultured at 37°C for 24 hours until full growth was achieved. A serial dilution of the cultured media was then performed, ranging from 2⁻¹ to 2⁻⁹. Each dilution was applied to screen-printed electrodes, covering them with 120 μl of the sample, and square wave voltammetry was conducted using the SIA potentiostat (Zimmer and Peacock, 2024) under the following conditions: Estep 0.01 V, amplitude 0.001 V, and frequency 1 Hz.